Genetic features and molecular epidemiology of Enterococcus faecium isolated in two university hospitals in Brazil
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- 作者：Leila Priscilla Pinheiro da Silva ; Andr¨¦ Pitondo-Silva ; Roberto Martinez ; Ana L¨²cia da Costa Darini
- 关键词：CC17 ; E. faecium ; MLST ; PFGE ; Tn1546 ; Virulence factors ; VREs
- 刊名：Diagnostic Microbiology and Infectious Disease
- 出版年：2012
- 出版时间：November, 2012
- 年：2012
- 卷：74
- 期：3
- 页码：267-271
- 全文大小：675 K
文摘
The global emergence of vancomycin-resistant Enterococcus faecium (VREfm) has been characterized by a clonal spread of strains belonging to clonal complex 17 (CC17). Genetic features and clonal relationships of 53 VREfm isolated from patients in 2 hospitals in Ribeirao Preto, S?o Paulo, Brazil, during 2005-2010 were determined as a contribution to the Brazilian evolutionary history of these nosocomial pathogens. All isolates were daptomycin susceptible, vancomycin-resistant, and had the vanA gene. The predominant virulence genes were acm and esp. Only 5 VREfm isolated in 2005-2006 had intact Tn1546, while 81 % showed Tn1546 with deleted left extremity and insertion of IS1251 between the vanS and vanH genes. Multilocus sequence typing analysis permitted the identification of 9 different sequence types (STs), with 5 being new ones (656, 657, 658, 659, and 660). Predominant STs were ST412 and ST478, all belonging to CC17, except ST658. This is the first report of the ST78 in Brazil.