Genetic identification in endodontic treated tooth root

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• 作者：A.T. Corte-Real ; M.J. Anjos ; L. Andrade ; M. Carvalho ; A. Serra ; A.M. Bento ; C. Oliveira ; L. Batista ; F. Corte-Real ; D.N. Vieira ; J.J. Gamero
• 关键词：Identification ; STR profile ; Endodontic tooth root
• 刊名：Forensic Science International: Genetics Supplement Series
• 出版年：2008
• 出版时间：August 2008
• 年：2008
• 卷：1
• 期：1
• 页码：457-458
• 全文大小：95 K

文摘

The tooth is an important sample in adverse forensic conditions. Its morphology and histology contributes for the preservation of the cellular components.

The endodontic root filling is a methodology used for preservation of tooth, in life, supporting a fixed rehabilitation. During endodontic procedure the pulp tissue and the inner layer of dentine are removed (the major nuclear DNA) and replaced for endodontic filling usually thermoplastic and cement. In the dentinal tubules, the odontoblats prolongations are preserved (mitochondrial DNA).

In this paper were studied endodontic tooth and the respective donor blood on compresses as reference samples. The samples were prepared. Teeth DNA extraction was made with a commercial kit, Purigene^® DNA Purification System (PE Gentra), and quantified with an ABI Prism^® 7000; amplification was performed with Identifiler™ PCR Amplification Kit (Applied Biosystems). In samples without STR profile was sequenced HVRI and HVRII. The samples were genotyped using an ABI Prism^® 310 and 3130 Analysers.

Results showed that with endodontic treated teeth were obtained STR profiles. Teeth are extraordinary samples in identification caseworks.

This study showed that even in roots with endodontic treatment were obtained full STR profiles, giving the possibility of individual genetic identification.