Development and validation of novel fiber relevant dbEST-SSR markers and their utility in revealing genetic diversity in diploid cotton (Gossypium herbaceum and G. arboreum)
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- 作者：Mithil J. Parekha ; 1 ; Sushil Kumara ; 1 ; sushil254386@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Harshvardhan N. Zalaa ; Ranbir S. Fougata ; Chandni B. Patela ; Tejas C. Bosamiab ; Kalyani S. Kulkarnia ; c ; Akarsh Parihara
- 关键词：Diploid cotton ; Diversity ; EST&ndash ; SSR ; SSR mining
- 刊名：Industrial Crops & Products
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：83
- 期：Complete
- 页码：620-629
- 全文大小：1719 K
文摘
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Compared to tetraploid, diploid cotton are poor in genomic resources including molecular markers.
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Sequence assembly and clustering of 1,31,464 database ESTs (G. hirsutum, 92,570 and G. arboreum, 38,894) resulted in 28,718 unigenes having 1,760 SSRs.
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A total of 378 novel primers were developed from the ESTs of cotton.
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A set of 60 primers associated with fiber development were validated on a panel of 32 genotypes.
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The UPGMA clustering grouped 32 genotypes in 2 clusters at cut off value of 0.23.