Bench scale DA pretreatment optimization of Cotton Stalk using RSM was conducted.
Conditions predicted by the model were validated by experimentation.
Correlation between combined severity factor with xylan and inhibitors was drawn.
The mass closure of the overall process was achieved within 6% error.
91.06% of the total saccharification yield was obtained.
The optimized conditions may be replicated in higher scale.
Physicochemical properties were evaluated on native and pretreated cotton stalk.