Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2 − in LPS/IFNγ activated glioma cells
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- 作者：Elizabeth A Mazzio ; David Bauer ; Patricia Mendonca ; Equar Taka ; Karam F.A. Soliman ; karam.soliman@famu.edu
- 关键词：C6 glioma ; Astrocytes ; Natural products ; HTP ; High throughput screening ; Herbs
- 刊名：Journal of Neuroimmunology
- 出版年：2017
- 出版时间：15 January 2017
- 年：2017
- 卷：302
- 期：Complete
- 页码：10-19
- 全文大小：1718 K
- 卷排序：302
文摘
Central nervous system (CNS) inflammation is often associated with neurodegenerative diseases and malignant glioblastoma. C6 glioma cells were evaluated for lipopolysaccharide (LPS)/ Interferon gamma (IFNγ) regulation of cytokines using an antibody array. Differential shifts were evident for MCP-1, NO2− and cytokine-induced neutrophil chemoattractants (CINC) 1, CINC 2a and CINC 3. Over 1400 natural products were evaluated for capacity to reduce NO2− in fully viable cells and only 1.77% showed anti-inflammatory effects. Of these, many also demonstrated significant attenuation of CINC-3 determined by ELISA. Lead products included: Ashwaganda (Withania somnifera), Elecampagne Root (Inula helenium), Feverfew (Tanacetum parthenium), Green Tea (Camellia sinensis), Turmeric Root (Curcuma Longa) Ganthoda (Valeriana wallichii), Tansy (Tanacetum vulgare), Maddar Root (Rubia tinctoria), Red Sandle wood (Pterocarpus santalinus), Bay Leaf (Laurus nobilis, Lauraceae), quercetin, cardamonin, fisetin, EGCG, biochanin A, galangin, apigenin and curcumin.