Molecular beacons with JOE dye: Influence of linker and 3′ couple quencher
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- 作者：Dmitry A. Tsybulskya ; b ; Maksim V. Kvachb ; Dmitry Y. Ryazantseva ; Ilya O. Aparina ; aparinmgu@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Alexander A. Stakheeva ; Igor A. Prokhorenkoa ; c ; Yury V. Martynenkob ; Sergey V. Gontarevd ; Andrey A. Formanovskya ; Timofei S. Zatsepine ; f ; g ; Vadim V. Shmanaib ; Vladimir A. Korshuna ; c ; Sergey K. Zavrieva ; szavriev@ibch.ru" class="auth_mail" title="E-mail the corresponding author
- 关键词：Real-time PCR ; Molecular beacons ; JOE dye ; Linkers ; Couple quencher
- 刊名：Molecular and Cellular Probes
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：30
- 期：5
- 页码：285-290
- 全文大小：1661 K
文摘
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A series of Molecular beacons with different structure carried JOE was tested in qPCR.
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JOE proved to be brighter than FAM with probes with the same sequence.
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The length of MB's stem was varied from 4 to 8 bp to optimize MBs design.
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A rigid linker for JOE attaching is preferable for increasing light-up of fluorescence.
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The second BHQ1 in probe significantly decrease background of fluorescence.