Immunogenicity of a plant-derived edible chimeric EspA, Intimin and Tir of Escherichia coli O157:H7 in mice

详细信息    查看全文

• 作者：Jafar Amani^a ; ^b ; ^{jamani@nigeb.ac.ir} ; Seyed Latif Mousavi^c ; ^{slmousavi@shahed.ac.ir} ; Sima Rafati^d ; ^{sima-rafatisy@pasteur.ac.ir} ; Ali Hatef Salmanian^a ; ^{salman@nigeb.ac.ir}
• 关键词：Escherichia coli O157 ; H7 ; Transgenic plants ; Type III secreted proteins ; Vaccine
• 刊名：Plant Science
• 出版年：2011
• 出版时间：April 2011
• 年：2011
• 卷：180
• 期：4
• 页码：620-627
• 全文大小：441 K

文摘

Transgenic plants offer the possibility to produce and deliver an oral immunogen on a large-scale with low production costs and minimal purification or enrichment. Cattles are important reservoirs of Escherichia coli O157:H7 and developing a specific immunity in animals would be invaluable. Intimin, Tir, and EspA proteins are the virulence factors expressed by LEE locus of enterohemorrhagic E. coli. We hypothesized that the chimeric recombinant forms of these effectors delivered as an edible-base vaccine would reduce colonization of bacteria in mice. A synthetic gene (eit) composed of espA (e), eae (i) and tir (t) attached by linkers was constructed. The gene was codon optimized and cloned into plant expression vectors adjacent to CaMV35S and FAE promoters for expression in tobacco and canola plants. Of total soluble protein 0.2 % and 0.3 % (in average) were detected in transgenic tobacco leaves and canola seeds respectively. Mice immunized either subcutaneously or orally with recombinant EIT and challenged with E. coli O157:H7 significantly exhibited reduced bacterial shedding. Application of transgenic plants containing trivalent immunogen is an effective tool for protection against E. coli O157:H7.