Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer
详细信息 查看全文
- 作者：Qinxin Song ; Guijiang Wei ; Guohua Zhou
- 关键词：GMO ; genetically modified organism ; IRMM ; Institute for Reference Materials and Measurements ; Bt11 and Bt176 ; genetically modified maize lines ; zSSIIb ; Zea mays starch synthase isoform zSTSII-2 ; EPSPS ; a region of 5-enolpyruvylshikimate-3-phosphate synthase gene introduced into NK603 ; CaMV ; cauliflower mosaic virus ; 35S ; 35S promoter region derived from CaMV ; NOS ; terminator region of the nopaline synthase gene derived from Rhizobium radiobacter ; PCR ; polymerase chain reaction ; PD ; photoallergy
- 刊名：Food Chemistry
- 出版年：1 July, 2014
- 年：2014
- 卷：154
- 期：Complete
- 页码：78-83
- 全文大小：1149 K
文摘
A portable bioluminescence analyser for detecting the DNA sequence of genetically modified organisms (GMOs) was developed by using a photodiode (PD) array. Pyrosequencing on eight genes (zSSIIb, Bt11 and Bt176 gene of genetically modified maize; Lectin, 35S-CTP4, CP4EPSPS, CaMV35S promoter and NOS terminator of the genetically modified Roundup ready soya) was successfully detected with this instrument. The corresponding limit of detection (LOD) was 0.01% with 35 PCR cycles. The maize and soya available from three different provenances in China were detected. The results indicate that pyrosequencing using the small size of the detector is a simple, inexpensive, and reliable way in a farm/field test of GMO analysis.