Optimized verification method for detection of an albumin-sulfur mustard adduct at Cys34 using a hybrid quadrupole time-of-flight tandem mass spectrometer after direct plasma proteolysis
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- 作者：Harald Johna ; HaraldJohn@bundeswehr.org" class="auth_mail" title="E-mail the corresponding author ; Markus Siegertb ; Felix Gandorc ; Michael Gawlikd ; Andreas Kranawetvogla ; Konstantin Karaghiosoffb ; Horst Thiermanna
- 关键词：Albumin-adducts ; High resolution mass spectrometry ; Hydroxyethylthioethyl ; Pronase ; Verification analysis ; Vesicant
- 刊名：Toxicology Letters
- 出版年：2016
- 出版时间：26 February 2016
- 年：2016
- 卷：244
- 期：Complete
- 页码：103-111
- 全文大小：902 K
文摘
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Sulfur mustard is a banned chemical warfare agent alkylating serum albumin.
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Direct plasma proteolysis with pronase yielded HETE-CP as biomarker for SM-exposure.
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A high resolving hybrid mass spectrometer provided excellent selectivity and sensitivity.
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The novel procedure proved applicability to real samples of poisoning.