Engineering cytosolic acetyl-coenzyme A supply in Saccharomyces cerevisiae: Pathway stoichiometry, free-energy conservation and redox-cofactor balancing
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- 作者：Harmen M. van Rossum ; Barbara U. Kozak ; Jack T. Pronk ; Antonius J.A. van Maris ; A.J.A.vanMaris@tudelft.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：A-ALD ; acetylating acetaldehyde dehydrogenase ; acetyl-CoA ; acetyl coenzyme A ; acetyl-P ; acetyl-phosphate ; Ach1 ; CoA-transferase ; ACL ; ATP-citrate lyase ; ACS ; acetyl-CoA synthetase ; ADH ; alcohol dehydrogenase ; ALD ; acetaldehyde dehydrogenase ; CAT ; carnitine acetyltransferase ; CIT ; citrate synthase ; CoA ; coenzyme A ; E(rythrose-)4P ; erythrose-4-phosphate ; F1 ; 6P ; fructose-1 ; 6-biphosphate ; F6P ; fructose-6-phosphate ; FDH ; formate dehydrogenase ; FeS ; iron-sulfur ; FPR ; flavodoxin-NADP+ reductase ; fructo
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：36
- 期：Complete
- 页码：99-115
- 全文大小：1144 K
文摘
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Precursor supply strongly influences the yield of acetyl-CoA-derived products on sugars.
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The native yeast pathway for cytosolic acetyl-CoA formation is ATP inefficient.
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Pathways from glucose to acetyl-CoA have different ATP, NAD(P)H and carbon stoichiometries.
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Optimal pathway configuration for acetyl-CoA generation is strongly product dependent.
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Combinations of acetyl-CoA forming reactions should be considered in pathway design.