Interaction of cationic antimicrobial peptides with Mycoplasma pulmonis
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- 作者：Ho Jin Park ; Ki Mo Kang ; Kevin Dybvig ; Bok Luel Lee ; Yong Woo Jung ; In Hee Lee
- 关键词：AMP ; antimicrobial peptide ; POPC ; 1-palmytoyl-2-oleoyl l-¦Á-phosphatidylcholine ; POPG ; 1-palmytoyl-2-oleoyl l-¦Á-phosphatidylglycerol ; CL ; cardiolipin ; SPM ; sphingomyelin ; NaP ; sodium phosphate ; PI ; propidium iodine ; SEM ; scanning electron microscopy ; TLC
- 刊名：FEBS Letters
- 出版年：2013
- 出版时间：11 October, 2013
- 年：2013
- 卷：587
- 期：20
- 页码：3321-3326
- 全文大小：983 K
文摘
We investigated the mode of action underlying the anti-mycoplasma activity of cationic antimicrobial peptides (AMPs) using four known AMPs and Mycoplasma pulmonis as a model mycoplasma. Scanning electron microscopy revealed that the integrity of the M. pulmonis membrane was significantly damaged within 30 min of AMPs exposure, which was confirmed by measuring the uptake of propidium iodine into the mycoplasma cells. The anti-mycoplasma activity of AMPs was found to depend on the binding affinity for phosphatidylcholine, which was incorporated into the mycoplasma membrane from the growth medium and preferentially distributed in the outer leaflet of the lipid bilayer.