Engineering of Harobin for enhanced fibrinolytic activity obtained by random and site-directed mutagenesis
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- 作者：Zhuojian Lia ; 1 ; Xiaojia Chena ; 1 ; Shujun Guoa ; Huihua Zhanga ; Haojun Donga ; Guoyi Wua ; An Honga ; tha@jnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jun Gub ; gj@pku.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Random mutagenesis ; Double mutant ; Harobin ; Thrombosis ; Hypertension
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：162-172
- 全文大小：2167 K
文摘
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Random- and site-directed mutagenesis were performed to enhance the fibrinolytic activity of serine protease Harobin.
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A Harobin double mutant, N111R/R230G, was constructed and can be functionally expressed in laboratory fermenter.
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It possessed much higher fibrinolytic activity and anti-thrombosis effect than wild type enzyme and other single mutants.