Proteinase-activated receptor 2 expression in the intestinal tract of the horse

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• 作者：Augusta Zannoni ; Cristiano Bombardi ; Francesco Dondi ; Maria Morini ; Monica Forni ; Roberto Chiocchetti ; Alessandro Spadari ; Noemi Romagnoli ; ^{noemi.romagnoli@unibo.it" class="auth_mail}
• 关键词：Horse ; Proteinase-activated receptor 2 ; Jejunum ; Ileum ; Pelvic flexure
• 刊名：Research in Veterinary Science
• 出版年：June 2014
• 年：2014
• 卷：96
• 期：3
• 页码：464-471
• 全文大小：1845 K

文摘

Proteinase-activated receptor 2 (PAR₂) is a G-protein-coupled receptor for trypsin and mast cell tryptase; it is highly expressed at the intestinal level with multiple functions, such as epithelial permeability and intestinal motility. Many proteases activate PAR₂ during tissue damage, suggesting a role of the inflammatory response receptors. The aim of the study was to evaluate the distribution and expression of PAR₂ in the jejunum, the ileum and the pelvic flexure, using samples collected from healthy adult horses after slaughter. Proteinase-activated receptor 2 immunoreactivity (PAR₂-IR) was observed in the enterocytes, intestinal glands, the smooth muscle of the muscularis mucosae, and the longitudinal and circular muscle layers; there were no differences in the distribution of PAR₂-IR in the different sections of the intestinal tract. The protein expression level showed that the relative amount of the PAR₂ content in the mucosa of the intestinal tract decreased from the small to the large intestine while the PAR₂ mRNA analysed showed similar values. This study provides relevant findings concerning the distribution of the PAR₂ in the intestines of healthy horses and represents the starting point for evaluating the role of the PAR₂ during strangulative intestinal disease and consequent systemic intestinal reperfusion/injury complications in horses in order to identify and employ antagonist PAR₂ molecules.