Absence of peripheral microchimerism following HLA- or non-HLA-sharing pretransplantation blood transfusions.

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• 作者：Caillat-Zucman ; S ; Benini ; V
• 刊名：Human Immunology
• 出版年：6 -,
• 出版时间：April, 15 1996 -,
• 年：0
• 卷：47
• 期：1-2
• 页码：164
• 全文大小：121 K

文摘

The beneficial effect of pretransplantation blood transfusions is still controversial. The cellular mechanisms underlying immune tolerance remain elusive, although transfusions have been reported to be associated with reduced alloimmunization and better protection from acute allograft rejection episodes. To investigate whether the development of a systemic microchimerism following transfusion could account for such observations, the presence of peripheral donor-recipient microchimerism was investigated in two groups of hemodialyzed patients given two different pregrafting transfusion protocols. Patients received one unit of third-party whole blood without HLA-DR identity (group 1, n=22) or with one DR identity (group 2, n=19) as defined by a shared DRB1^* allele following generic genotyping. Peripheral blood microchimerism was studied 1, 4, 8 and 12 weeks after transfusion using sequence-specific amplification (detection threshold 40 pg DNA corresponding to a 1/4000 donor/recipient cell ratio). A transient microchimerism was observed at 1 and/or 4 weeks after transfusion in 8 patients (4 in each group), and disappeared afterwards. These results do not support a major role for the establishment of microchimerism in the induction of tolerance following pregrafting transfusion.