Pink1/Parkin-mediated mitophagy play a protective role in cisplatin induced renal tubular epithelial cells injury

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• 作者：Chuanyan Zhao^a ; ¹ ; Zhuyun Chen^a ; ¹ ; Xueqiang Xu^a ; Xiaofei An^b ; Suyan Duan^a ; Zhimin Huang^a ; Chengning Zhang^a ; Lin Wu^a ; Bo Zhang^a ; Aihua Zhang^c ; ^d ; Changying Xing^a ; ^{cyxing62@126.com} ; Yanggang Yuan^a ; ^{ygyuan@njmu.edu.cn}
• 关键词：Pink1 ; Parkin ; Mitophagy ; Mitochondrial dysfunction ; Cisplatin ; AKI
• 刊名：Experimental Cell Research
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：350
• 期：2
• 页码：390-397
• 全文大小：1193 K
• 卷排序：350

文摘

Cisplatin often causes acute kidney injury (AKI) in the treatment of a wide variety of malignancies. Mitochondrial dysfunction is one of the main reasons for cisplatin nephrotoxicity. Previous study showed that Pink1 and Parkin play central roles in regulating the mitophagy, which is a key protective mechanism by specifically eliminating dysfunctional or damaged mitochondria. However, the mechanisms that modulate mitophagy in cisplatin induced nephrotoxicity remain to be elucidated. The purpose of this study was to investigate the effects of Pink1/Parkin pathway in mitophagy, mitochondrial dysfunction and renal proximal tubular cells injury during cisplatin treatment. In cultured human renal proximal tubular cells, we found that knockdown of Pink1/Parkin induced the aggravation of mitochondrial function, leading to the increase of cell injury through inhibition of mitophagy. Additionally, the overexpression of Pink1/Parkin protected against cisplatin-induced mitochondrial dysfunction and cell injury by promoting mitophagy. Our results provide clear evidence that Pink1/Parkin-dependent mitophagy has identified potential targets for the treatment of cisplatin-induced AKI.