A high-throughput, in-vitro assay for Bacillus thuringiensis insecticidal proteins
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- 作者：Michi Izumi Willcoxon ; michi.izumi@pioneer.com" class="auth_mail" title="E-mail the corresponding author ; Jaclyn R. Dennis ; Sabina I. Lau ; Weiping Xie ; You You ; Song Leng ; Ryan C. Fong ; Takashi Yamamoto
- 关键词：Bt ; Bacillus thuringiensis ; BBMV ; brush border membrane vesicles ; On ; Ostrinia nubilalis ; Sf ; Spodoptera frugiperda ; Cad ; cadherin-like protein ; APN ; aminopeptidase N ; MoA ; mode of action ; GUS ; β-glucuronidase
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：217
- 期：Complete
- 页码：72-81
- 全文大小：1311 K
文摘
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High-throughput assay method development for Bacillus thuringiensis (Bt) insecticidal proteins.
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The method utilizes an insect cell line expressing a functional receptor for Bt insecticidal proteins.
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The cell responses to the insecticidal proteins are measured by a chemical cell viability test kit.
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The assay can be done in 96-well microtiter plates to screen a large number of samples.