Detection of subtle differences in analogous viral capsid proteins by allowing unrestricted specific interaction in solution competition ELISA

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• 作者：Lu Cao^a ; ^b ; ^{caolu@stu.xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Xin Wang^a ; ^b ; ^{wxinc35@163.com" class="auth_mail" title="E-mail the corresponding author} ; Mujin Fang^a ; ^b ; ^{mjfang@xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Ningshao Xia^a ; ^b ; ^c ; ^{nsxia@xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Qinjian Zhao^a ; ^b ; ^{qinjian_zhao@xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antigen-antibody interaction ; Hepatitis E virus ; Viral capsid protein ; Solution interaction ; Binding analysis ; Monoclonal antibody
• 刊名：Journal of Virological Methods
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：236
• 期：Complete
• 页码：1-4
• 全文大小：1065 K

文摘

Assay artifacts were reported in plate-based immuoassays during the assessment of specific molecular interactions owing to the surface induced aggregation/conformational changes. To circumvent surface adsorption and associated artifacts, we used a solution competition ELISA by allowing unrestricted interaction between binding partners to occur in solution for better discrimination between epitopes with subtle differences. A difference of two orders of magnitude in binding to neutralizing antibodies for two truncated versions of the hepatitis E virus capsid protein was observed, while other assays showed comparable antigenicity with the same monoclonal antibodies. Discrimination of epitopes with high degree resemblance in analogous viral capsid proteins was demonstrated quantitatively based on their specific interactions. Therefore, the solution competition ELISA is a method of choice when the detection of subtle differences of two highly analogous proteins is desired.