Identifying gp85-regions involved in Epstein–Barr virus binding to B-lymphocytes

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• 作者：Urquiza ; Mauricio ; Suarez ; Jorge ; Lopez ; Ramses ; Vega ; Erika ; Patino ; Helena ; Garcia ; Javier ; et. al.
• 关键词：EBV ; gp85 ; Raji-cell binding peptides ; HABPs
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2004
• 出版时间：June 18, 2004
• 年：2004
• 卷：319
• 期：1
• 页码：221-229
• 全文大小：495 K

文摘

Epstein–Barr virus lacking glycoprotein gp85 cannot infect B-cells and epithelial cells. The gp85 belongs to the molecular complex required for virus invasion of B-lymphocyte or epithelial cells. Moreover, there is evidence that gp85 is necessary for virus attachment to epithelial cells. Thirty-six peptides from the entire gp85-sequence were tested in epithelial and lymphoblastoid cell line binding assays to identify gp85-regions involved in virus–cell interaction. Five of these peptides presented high binding activity to Raji, Ramos, P3HR-1, and HeLa cells, but not to erythrocytes; Raji-cell affinity constants were between 80 and 140nM. Of these five peptides, 11435 (¹⁸¹TYKRVTEKGDEHVLSLVFGK²⁰⁰), 11436 (²⁰¹TKDLPDLRGPFSYPSLTSAQ²²⁰), and 11438 (²⁴¹YFVPNLKDMFSRAVTMTAAS²⁶⁰) bound to a 65kDa protein on Raji-cell surface. These peptides and antibodies induced by them (recognising live EBV-infected cells) inhibited Epstein–Barr virus interaction with cord blood lymphocytes. It is thus probable that gp85-regions defined by peptides 11435, 11436, and 11438 are involved in EBV invasion of B-lymphocytes.