Magnetically softened iron oxide (MSIO) nanofluid and its application to thermally-induced heat shock proteins for ocular neuroprotection

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• 作者：Seongtae Bae^a ; ¹ ; ^{bae4@cec.sc.edu" class="auth_mail" title="E-mail the corresponding author} ; Jin Wook Jeoung^b ; ¹ ; Minhong Jeun^c ; Jung-tak Jang^a ; Joo Hyun Park^b ; Yu Jeong Kim^b ; Kwan Lee^a ; ^e ; Minkyu Kim^d ; ^e ; Jooyoung Lee^d ; ^e ; Hey Min Hwang^d ; ^e ; Sun Ha Paek^d ; ^e ; Ki Ho Park^b ; ^{kihopark@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Magnetic nanofluid hyperthermia (MNFH) ; Magnetically softened iron oxide (MSIO) ; (Mn0.5Zn0.5)Fe2O4 nanoparticles ; Heat shock proteins ; Ocular neuroprotection
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：101
• 期：Complete
• 页码：165-175
• 全文大小：2764 K

文摘

Magnetically softened iron oxide (MSIO) nanofluid, PEGylated (Mn_0.5Zn_0.5)Fe₂O₄, was successfully developed for local induction of heat shock proteins (HSPs) 72 in retinal ganglion cells (RGCs) for ocular neuroprotection. The MSIO nanofluid showed significantly enhanced alternating current (AC) magnetic heat induction characteristics including exceptionally high SLP (Specific Loss Power, > 2000 W/g). This phenomenon was resulted from the dramatically improved AC magnetic softness of MSIO caused by the magnetically tailored Mn²⁺ and Zn²⁺ distributions in Fe₃O₄. In addition, the MSIO nanofluid with ultra-thin surface coating layer thickness and high monodispersity allowed for a higher cellular uptake up to a 52.5% with RGCs and enhancing “relaxation power” for higher AC heating capability. The RGCs cultured with MSIO nanofluid successfully induced HSPs 72 by magnetic nanofluid hyperthermia (MNFH). Moreover, it was interestingly observed that systematic control of “AC magnetically-induced heating up rate” reaching to a constant heating temperature of HSPs 72 induction allowed to achieve maximized induction efficiency at the slowest AC heating up rate during MNFH. In addition to in-vitro experimental verification, the studies of MSIO infusion behavior using animal models and a newly designed magnetic coil system demonstrated that the MSIO has promising biotechnical feasibility for thermally-induced HSPs agents in future glaucoma clinics.