SGT1 interacts with the Prf resistance protein and is required for Prf accumulation and Prf-mediated defense signaling

详细信息    查看全文

• 作者：Joanna Kud^a ; ¹ ; Zhulu Zhao^b ; ¹ ; Xinran Du^a ; Yule Liu^c ; Yun Zhao^b ; Fangming Xiao^a ; ^{fxiao@uidaho.edu}
• 关键词：Tomato ; Nicotiana benthamiana ; SGT1 ; Co-chaperone ; Prf ; Protein accumulation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：431
• 期：3
• 页码：501-505
• 全文大小：536 K

文摘

The highly conserved eukaryotic co-chaperone SGT1 (suppressor of the G2 allele of skp1) is an important signaling component of plant defense responses and positively regulates disease resistance conferred by many resistance (R) proteins. In this study, we investigated the contribution of SGT1 in the Prf-mediated defense responses in both Nicotiana benthamiana and tomato (Solanum lycopersicum). SGT1 was demonstrated to interact with Prf in plant cells by co-immunoprecipitation. The requirement of SGT1 in the accumulation of Prf or autoactive Prf^D1416V was determined by the degradation of these proteins in N. benthamiana, in which SGT1 was repressed by virus-induced gene silencing (VIGS). Pseudomonas pathogen assay on the SGT1-silenced tomato plants implicates SGT1 is required for the Prf-mediated full resistance to Pseudomonas syringae pv. tomato (Pst). These results suggest that, in both N. benthamiana and tomato, SGT1 contributes to the Prf-mediated defense responses by stabilizing Prf protein via its co-chaperone activity.