CC-type glutaredoxins recruit the transcriptional co-repressor TOPLESS to TGA-dependent target promoters in Arabidopsis thaliana

详细信息    查看全文

• 作者：Joachim F. Uhrig ; Li-Jun Huang ; Sina Barghahn ; Moritz Willmer ; Corinna Thurow ; Christiane Gatz ; ^{cgatz@gwdg.de}
• 关键词：Arabidopsis thaliana ; Gene expression ; Glutaredoxins ; ROXYs ; TGA transcription factors ; TOPLESS
• 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1860
• 期：2
• 页码：218-226
• 全文大小：826 K
• 卷排序：1860

文摘

Glutaredoxin ROXY19 interacts simultaneously with TGA transcription factors and with the co-repressor TOPLESS. Mutating the conserved ALWL motif into an ALWA motif renders ROXY1 and ROXY19 unable to interact with TOPLESS. Mutating the ALWL motif into an ALWA motif renders ROXY19 unable to repress transcription. Proteins that compete with ROXY19 for binding to TOPLESS interfere with the repressive function of ROXY19. Our conclusion: ROXYs repress transcription by bridging TGA factors with TOPLESS at specific target promoters.