Expression of heat shock protein 27 and troponin T and troponin I after naloxone-precipitated morphine withdrawal
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- 作者：Elena Martí ; nez-Laorden ; Pilar Almela ; palmela@um.es" class="auth_mail" title="E-mail the corresponding author ; Marí ; a-Victoria Milané ; s ; Marí ; a-Luisa Laorden
- 关键词：BSA ; bovine serum albumin ; cTnT ; cardiac troponin T ; cTnI ; cardiac troponin I ; Hsp27 ; Heat shock protein 27 ; HR ; heart rate ; MAP ; mean arterial blood pressure ; PVDF ; poly vinylidene difluoride ; Ser82 ; serine82 ; SDS ; sodium dodecylsulfate ; TBST ; tris buffer saline tween
- 刊名：European Journal of Pharmacology
- 出版年：2015
- 出版时间：5 November 2015
- 年：2015
- 卷：766
- 期：Complete
- 页码：142-150
- 全文大小：2793 K
文摘
Heat shock protein (Hsp27) renders cardioprotection from stress situations but little is known about its role in myofilaments. In this study we have evaluated the relationship between Hsp27 and troponin response after naloxone-induced morphine withdrawal. Rats were treated with two morphine (75 mg) pellets during six days. Precipitated withdrawal was induced by naloxone on day seven. Hsp27 expression, Hsp27 phosphorylated at serine 82 (Ser82), cardiac troponin T (cTnT), cardiac troponin I (cTnI) and µ-calpain were evaluated by immunoblotting in left ventricle. Hsp, cTnT and cTnI was also evaluated by immunofluorescence procedure. Our results show that enhancement in Hsp27 expression and phosphorylation induced by naloxone-precipitated morphine withdrawal occurs with concomitant increases of cTnT and µ-calpain expression, whereas cTnI was decreased. We also observed co-localization of Hsp27 with cTnT in cardiac tissues. These findings provide new information into the possible role of Hsp27 in the protection of cTnT degradation by µ-calpain (a protease mediating proteolysis of cTnT and cTnI) after morphine withdrawal.