Thermal stress induces a distinct transcriptome profile in the Pacific oyster Crassostrea gigas

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• 作者：Hyun-Jeong Lim^a ; ¹ ; Bo-Mi Kim^b ; ¹ ; In Joon Hwang^a ; Jeong-Soo Lee^c ; Ik-Young Choi^c ; ^d ; ^{ikyoung02@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Youn-Jung Kim^e ; ^{duckyj@inu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Jae-Sung Rhee^e ; ^{jsrhee@inu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Oyster ; Crassostrea gigas ; Transcriptome ; Temperature ; Thermal stress
• 刊名：Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：19
• 期：Complete
• 页码：62-70
• 全文大小：1592 K

文摘

Oysters are frequently subjected to heat stress during tidal emersion/immersion cycles in their habitats due to attachment on the rocky shore. To understand the effect of temperature elevation on the whole transcriptome over time, the Pacific oyster Crassostrea gigas was exposed to seawater temperature 32 °C for 72 h from the control 20 °C. RNA-seq identified differentially expressed stress responsive transcripts upon thermal stress in the gill tissues of C. gigas. The primary effect of heat stress appears to be significantly induced transcription of molecular chaperones, including members of the heat shock protein (hsp) families, while genes typically associated with protein metabolism, such as those involved in protein degradation (e.g. ATP-dependent proteolysis pathway) and biosynthesis (e.g. ribosomal protein genes), were repressed. In particular, several hsp70 isoforms and a small hsp20 maintained prolonged mRNA expressions for 72 h. This study provides preliminary insights into the molecular response of C. gigas to heat stress and suggests a basis for future studies examining molecular adaptation or thermotolerance metabolism in the Pacific oyster.