Proteome identification of the silkworm middle silk gland

详细信息    查看全文

• 作者：Jian-ying Li^a ; ^b ; Lu-peng Ye^b ; Jia-qian Che^b ; Jia Song^b ; Zheng-ying You^b ; Shao-hua Wang^b ; Bo-xiong Zhong^b ; ^{bxzhong@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bombyx mori ; Middle silk gland ; Silk protein synthesis ; Shotgun proteomics ; Label-free
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：6
• 期：Complete
• 页码：903-907
• 全文大小：1197 K

文摘

To investigate the functional differentiation among the anterior (A), middle (M), and posterior (P) regions of silkworm middle silk gland (MSG), their proteomes were characterized by shotgun LC–MS/MS analysis with a LTQ-Orbitrap mass spectrometer. To get better proteome identification and quantification, triplicate replicates of mass spectrometry analysis were performed for each sample. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (Vizcaíno et al., 2014) [1] via the PRIDE partner repository (Vizcaino, 2013) [2] with the dataset identifier PXD003371. The peptide identifications that were further processed by PeptideProphet program in Trans-Proteomic Pipeline (TPP) after database search with Mascot software were also available in .XML format files. Data presented here are related to a research article published in Journal of Proteomics by Li et al. (2015) [3].