The biodegradation pathway of triethylamine and its biodegradation by immobilized Arthrobacter protophormiae cells

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• 作者：Tianming Cai^a ; Liwei Chen ; ^a ; ^{clw@njau.edu.cn} ; Qian Ren^a ; Shu Cai^a ; Jin Zhang^a
• 关键词：Triethylamine ; Arthrobacter protophormiae ; Biodegradation ; Immobilized cells
• 刊名：Journal of Hazardous Materials
• 出版年：2011
• 出版时间：15 February 2011
• 年：2011
• 卷：186
• 期：1
• 页码：59-66
• 全文大小：409 K

文摘

A bacterial strain named R4 was isolated from a wastewater treatment pool containing triethylamine (TEA) as the sole source of carbon and nitrogen. Strain R4 was identified as Arthrobacter protophormiae based on 16S rRNA gene sequence analysis and morphological and physiological properties. The optimal pH, temperature and concentration of NaCl for TEA degradation by strain R4 were 7.0, 30 °C and 0.5 % , respectively. Strain R4 could completely degrade 100 mg l⁻¹ TEA to ammonia in 32 h, and could also effectively degrade diethylamine (DEA) and ethylamine (EA) to ammonia. The degradation of TEA was strongly inhibited by some metal ions (Cu²⁺, Mn²⁺, Zn²⁺, Co²⁺, Ni²⁺ and Ag⁺) (1.0 mM). Addition of either SO₄²⁻ or NH₄⁺ reduced the degradation efficiency of TEA by strain R4 to a certain extent. The inhibition became significant when the concentration of SO₄²⁻ and NH₄⁺ reached to 11 mM and 30 mM, respectively. Cell-free extracts prepared from cells grown in TEA exhibited TEA monooxygenase, DEA monooxygenase and EA monooxygenase activity. Here, we propose the metabolic pathway of TEA degradation in strain R4. The efficiency of TEA removal by immobilized cells of strain R4 was found to be equivalent to that of free cells. In addition, the immobilized cells could be reused without reduction in their ability to degrade TEA.