Highly porous 3D nanofibrous scaffolds processed with an electrospinning/laser process

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• 作者：Min Seong Kim ; JoonGon Son ; HyeongJin Lee ; Heon Hwang ; Chang Hyun Choi ; GeunHyung Kim
• 关键词：Nanofiber ; Electrospinning ; Laser process ; Biomaterial
• 刊名：Current Applied Physics
• 出版年：January, 2014
• 年：2014
• 卷：14
• 期：1
• 页码：1-7
• 全文大小：3158 K

文摘

Electrospinning has been widely used to produce micro/nanosized fibres. Although the method is very simple, easy, and effective for obtaining nanosized material, the fabrication of three dimensional (3D) shapes comprised of micro/nanofibres has been a major obstacle for use in tissue engineering. In this study, a new electrospinning method to fabricate controllable 3D micro/nanofibrous structure (with thickness over 3聽mm) is suggested. The fabricated 3D fibrous structure was fully porous and successfully consisted of submicron-sized fibres. However, the pores in the 3D fibrous structure were too small (5-10聽渭m), so we used a femtosecond laser process to achieve enough cell infiltration and proliferation in the thickness direction of the 3D structure. By controlling appropriate processing conditions, we can successfully fabricate a highly porous 3D micro/nanofibrous structure with various pore sizes ranging from 189聽卤聽28聽渭m to 380聽卤聽21聽渭m. The fabricated 3D fibrous scaffolds were assessed for in聽vitro biological capabilities by culturing osteoblast like cells (MG63). Compared with the rapid-prototyped PCL scaffold, the 3D fibrous scaffold exhibited significantly higher biological activities (initial cell attachment and cell proliferation) due to the topographical structure of micro/nanofibres.