Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer
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- 作者：Michaela Frye ; Ilaria Dragoni ; Suet-Feung Chin ; Immaculada Spiteri ; Agata Kurowski ; Elena Provenzano ; Andrew Green ; Ian O. Ellis ; Donna Grimmer ; Andrew Teschendorff ; Christos C. Zouboulis ; Carlos Cald
- 关键词：RNA methyltransferase ; Myc ; Gene amplification ; Breast cancer
- 刊名：Cancer Letters
- 出版年：2010
- 出版时间：1 March 2010
- 年：2010
- 卷：289
- 期：1
- 页码：71-80
- 全文大小：801 K
文摘
We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.