Identification of pathogenicity, investigation of virulent gene distribution and development of a virulent strain-specific detection PCR method for Vibrio harveyi isolated from Hainan Province and Guangdong Province, China

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• 作者：Xiandong Xu^a ; ^b ; ^c ; Kaifang Liu^a ; Shifeng Wang^a ; Weiliang Guo^b ; Zhenyu Xie^b ; ^{xiezyscuta@163.com} ; Yongcan Zhou^a ; ^{zychnu@163.com}
• 关键词：Vibrio harveyi ; Molecular genotype ; Pathogenic strains ; Identification ; Virulence genes ; Specific primer
• 刊名：Aquaculture
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：468
• 期：part_P1
• 页码：226-234
• 全文大小：883 K
• 卷排序：468

文摘

This study found eighteen pathogenic Vibrio harveyi strains in south China had similar genetic backgrounds and might be representative pathogenic strains of this region. The pathogenic strains of V. harveyi could be specifically detected using ERIC-PCR fingerprint-based amplification. Strain-specific PCR primers were designed based on the sequences of specific bands amplified from pathogen strains of V. harveyi by ERIC-PCR fingerprinting.