Type 2 and 5 17β-hydroxysteroid dehydrogenases and androgen receptor in human fetal lungs

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• 作者：Marc Simard ; Julie Plante ; Mé ; lanie Boucher ; Pierre R. Provost ; Yves Tremblay
• 关键词：Prematurity ; Lung maturation ; Steroidogenesis ; Testosterone ; Housekeeping gene stability
• 刊名：Molecular and Cellular Endocrinology
• 出版年：2010
• 出版时间：5 May 2010
• 年：2010
• 卷：319
• 期：1-2
• 页码：79-87
• 全文大小：1685 K

文摘

Androgens delay fetal lung maturation through an androgen receptor (AR)-dependent mechanism. Type 2 and 5 17β-hydroxysteroid dehydrogenases (17βHSD) are involved in androgen inactivation and synthesis, respectively. We aimed to further characterize the human fetal lung potential for androgen metabolism and response. 17βHSD2, 17βHSD5, and AR mRNA levels were determined in lungs of mid–late gestation and in adult lungs, while protein detections were performed at mid-gestation. Relationships between levels of each mRNA and gestational age were observed. AR protein levels showed important differences among individuals of the same gestational window. 17βHSD2 and AR were co-localized in epithelial and mesenchymal cells. AR was detected in both, cytoplasm and nucleus, which suggests fine-tuning of AR occupancy. In contrast, 17βHSD5 was localized in a few epithelial cells of conducting zones. Our results support the existence of a local androgen metabolism in male and female human fetal lungs during the period of high-risk premature birth.