ELISA-PLA: A novel hybrid platform for the rapid, highly sensitive and specific quantification of proteins and post-translational modifications
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- 作者：Qing-He Tonga ; Tao Taoa ; Li-Qi Xiea ; xieliqi@fudan.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Hao-Jie Lua ; b ; luhaojie@fudan.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：ELISA ; Proximity ligation assay ; O-GlcNAcylation ; Phosphorylation
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 June 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：385-391
- 全文大小：1353 K
文摘
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We developed a rapid, specific and sensitive proteins and PTMs quantifying platform.
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Proximity ligation assay (PLA) was introduced in Sandwich ELISA.
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PLA improves specificity by proximate recognition through multiple probes.
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It shows two orders lower LOD and wider dynamic ranges than sandwich ELISA.
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It is faster and more sensitive than IP–WB in proteins PTMs detection.