HIV-1 Nef binds with human GCC185 protein and regulates mannose 6 phosphate receptor recycling
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- 作者：Manjeet Kumar ; Supinder Kaur ; Aamir Nazir ; Raj Kamal Tripathi ; rajkamalcdri@gmail.com" class="auth_mail" title="E-mail the corresponding author ; rajkamalt@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein&ndash ; protein interaction ; Caenorhadbitis elegans ; Human homolog ; Mammalian two hybrid assay ; In situ proximity ligation assay ; Regulation of MPRs cycle ; Late endosome trafficking ; Lysosome ; trans-Golgi network
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：20 May 2016
- 年：2016
- 卷：474
- 期：1
- 页码：137-145
- 全文大小：1300 K
文摘
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Nef, an accessory protein of HIV-1 interacts with host factor and culminates into AIDS pathogenesis.
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Using Caenorhabditis elegans based screen system, novel Nef interacting cellular protein GCC185 was identified.
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Molecular characterization of Nef and human protein GCC185 revealed Nef EEEE65 key region interacted with full length GCC185.
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Nef impeded the GCC185-Rab 9 interaction and perturb the mannose 6 phosphate receptor recycling.
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Our study identified novel Nef interacting human GCC185 protein and their role regulation of M6P receptor recycling.