Drug functionalized microbial polysaccharide based nanofibers as transdermal substitute

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• 作者：Priya Vashisth ; PhD^a ; Amit Kumar Srivastava ; MPharm^b ; Hemant Nagar ; MPharm^b ; Navdeep Raghuwanshi ; MTech^a ; ^b ; Shruti Sharan ; MSc^a ; Kumar Nikhil ; PhD^a ; Parul A. Pruthi ; PhD^a ; Rajesh P. Singh ; PhD^a ; Partha Roy ; PhD^a ; Vikas Pruthi ; PhD^a ; ^{vikasfbs@iitr.ernet.in" class="auth_mail" title="E-mail the corresponding author} ; ^{vikasfbs@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Gellan ; Transdermal substitute ; Nanofibers ; Electrospinning ; Wound healing
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：12
• 期：5
• 页码：1375-1385
• 全文大小：2775 K

文摘

In order to promote the natural healing process, drug-functionalized nanofibrous transdermal substitute was fabricated using gellan as chief polymer and polyvinyl alcohol (PVA) as supporting polymer via electrospinning technique. These fabricated nanofibers physiochemically mimic the extracellular matrix (ECM) which supports the cell growth. For neo-tissue regeneration in a sterilized environment, amoxicillin (Amx) was entrapped within these nanofibers. Entrapment of Amx in the nanofibers was confirmed by FESEM, FTIR, XRD and TG analysis. In vitro cell culture studies revealed that the fabricated non-cytotoxic nanofibers promoted enhance cell adherence and proliferation of human keratinocytes. A preliminary in vivo study performed on rat model for full thickness skin excision wound demonstrated the prompt re-epithelialization in early phase and quicker collagen deposition in later phases of wound healing in case of Amx-functionalized gellan/PVA nanofibers. Data collectively confirmed the potential usage of gellan based electrospun nanofibers as transdermal substitute for faster skin restoration.