Mechanisms of enhanced carbohydrate and lipid metabolism in adipose tissue in uremia

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• 作者：Marek Szolkiewicz ; MD ; PhD^* ; Elzbieta Sucajtys ; MSc^* ; Wojciech Wolyniec ; MD^* ; Przemyslaw Rutkowski ; MD ; PhD^* ; Ewa Stelmanska ; MSc^&dagger ; ; Justyna Korczynska ; MSc^&dagger ; ; Julian Swierczynski ; MD ; PhD^&dagger ; ; Boleslaw Rutkowski ; MD ; PhD^* ; ^{bolo@amedec.amg.gda.pl}
• 刊名：Journal of Renal Nutrition
• 出版年：2005
• 出版时间：January 2005
• 年：2005
• 卷：15
• 期：1
• 页码：166-172
• 全文大小：159 K

文摘

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Objective

Hyperlipidemia is a permanent finding in advanced renal failure. It is supposed to be responsible for the accelerated arteriosclerosis and cardiovascular complications observed in patients with that disease. The background is partially determined, however, our knowledge in this matter is not yet satisfactory.

Methods

This study is based on the experimental rat model of chronic renal failure (CRF). Considering white adipose tissue (WAT) lipogenesis upregulation in CRF, along with the determination of acetyl coenzyme A carboxylase (ACC) and fatty acid synthase (FAS) genes expression, we have measured WAT gene expression for sterol regulatory binding protein 1 (SREBP-1) at the level of protein mass and mRNA abundance. Furthermore, we have determined glucose uptake, glucose-to-CO₂ conversion rate, and glucose translocator (GLUT-4) gene expression in WAT.

Results

Upregulation of both FAS and ACC gene expression was found in WAT of CRF rats. It was accompanied by WAT SREBP-1 gene overexpression. Moreover, we have observed the increased glucose uptake, glucose to CO₂ conversion rate, and GLUT-4 gene expression in WAT of CRF rats in comparison with controls.

Conclusion

SREBP-1 gene overexpression may contribute to enhanced lipogenesis upregulation in WAT of CRF rats. It is not excluded that the increased WAT glucose metabolism activity is also induced by this mechanism, although there is no evidence currently to that end. We hypothesize that the increased WAT lipogenesis capacity could be a part of mechanism(s) leading to CRF-induced hyperlipidemia.