Would calcium or potassium channels be responsible for cardiac arrest produced by adenosine and ATP in the right atria of Wistar rats?

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• 作者：Henrique Camara^a ; ¹ ; Juliano Quintella Dantas Rodrigues^a ; ¹ ; ^{juliano.quintella@unifesp.br" class="auth_mail" title="E-mail the corresponding author} ; Gabriel Andrade Alves^b ; Edilson Dantas da Silva Junior^a ; Afonso Caricati-Neto^a ; Antô ; nio G. Garcia^c ; Aron Jurkiewicz^a ; ^{aron.farm@epm.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：N6-Cyclopentyladenosine (PubChem CID ; 657378) ; IB-MECA (PubChem CID ; 123683) ; CGS21680 (PubChem CID ; 3086599) ; BayK 8644 (PubChem CID ; 2303) ; ATPγS (PubChem CID ; 440317) ; DPCPX (PubChem CID ; 1329) ; MRS 1220 (PubChem CID ; 393595) ; ZM 241385 (PubChem CID ; 176407) ; 4-Aminopyridine (PubChem CID ; 444229) ; MRS2179 (PubChem CID ; 5311302)
• 刊名：European Journal of Pharmacology
• 出版年：2015
• 出版时间：5 December 2015
• 年：2015
• 卷：768
• 期：Complete
• 页码：199-206
• 全文大小：1553 K

文摘

Autonomic nerves release ATP, which is processed into adenosine in the synaptic cleft. Adenosine and ATP exert a negative chronotropic effect in the heart. This study aims to evaluate adenosine and P2 receptors and cellular signalling in cardiac arrest produced by purines in the heart. Right atria of adult Wistar rats were used to evaluate the effects of adenosine, ATP and CPA (an adenosine A₁ receptor agonist), in the presence and absence of DPCPX, an adenosine A₁ receptor antagonist. Effects of adenosine A₂ and A₃ receptors agonists and antagonists were also investigated. Finally, involvement of calcium and potassium channels in these responses was assessed using BayK 8644 and 4-Aminopyridine. Cumulative concentration–effect curves of adenosine and CPA resulted in a negative chronotropic effect culminating in cardiac arrest at 1000 μM (adenosine) and 1 µM (CPA). Furthermore, ATP produced a negative chronotropic effect at 1–300 µM and cardiac arrest at 1000 μM in the right atrium. ATPγS (a non-hydrolysable analogue of ATP) reduced chronotropism only. The effects of adenosine, CPA and ATP were inhibited by DPCPX, a selective adenosine A₁ receptor antagonist. The selective adenosine A₂ and A₃ receptors antagonists did not alter the chronotropic response of adenosine. 4-Aminopyridine, a blocker of potassium channels at 10 mM, prevented the cardiac arrest produced by adenosine and ATP, while BayK 8644, activator of calcium channels, did not prevent cardiac arrest. Adenosine A₁ receptor activation by adenosine and ATP produces cardiac arrest in the right atrium of Wistar rats predominantly through activation of potassium channels.