Lycium barbarum polysaccharides attenuates N-methy-N-nitrosourea-induced photoreceptor cell apoptosis in rats through regulation of poly (ADP-ribose) polymerase and caspase expression

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• 作者：Yafei Zhu^a ; ^b ; Qipeng Zhao^a ; ^b ; Hua Gao^c ; Xiaodong Peng^a ; ^b ; Youmin Wen^c ; ^{nxwym@163.com" class="auth_mail" title="E-mail the corresponding author} ; Guidong Dai^d ; ^{daiguidong@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：N-Methyl-N-nitrosourea (PubChem CID ; 12699) ; Hematoxylin (PubChem CID ; 442514) ; Eosin (PubChem CID ; 11048) ; N ; N'-Methylenebisacrylamide (PubChem CID ; 8041) ; Tris base (PubChem CID ; 6503) ; SDS (PubChem CID ; 3423265) ; DAPI (PubChem CID ; 2954) ; Formalin (PubChem CID ; 712) ; PMSF (PubChem CID ; 4784) ; Dithiothreitol (PubChem CID ; 446094)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：15 September 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：125-134
• 全文大小：2862 K

文摘

Lycium barbarum L., popularly known as “Goji berry”, a classic of Traditional Chinese Medicine has long been used to treat ocular diseases and cardiovascular diseases. Recently, the photoreceptor cell protection of Lycium barbarum polysaccharides (LBP), a water extract from Lycium barbarum L. has received more attention. The present study was designed to investigate the effect of LBP on N-methyl-N-nitrosourea (MNU)-induced photoreceptor cell apoptosis, and the involvement of the poly (ADP-ribose) polymerase (PARP) and caspase.

Materials and methods

Photoreceptor cell injury was induced in male Sprague-Dawley rats by an intraperitoneal injection of MNU 60 mg/kg. Seven days prior to MNU injection, LBP were intragastrical administered daily, rats were sacrificed at 24 h and 7 days after MNU injection. Retinal morphologies, photoreceptor cells apoptosis, and protein expression were evaluated at 24 h and 7 days after MNU injection.

Results

Morphologically, the outer nuclear layer was well preserved in the LBP-treated rat retinas throughout the experimental period. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-digoxigenin nick-end labeling (TUNEL) assays showed that LBP could significantly suppress the loss of photoreceptor cells, as determined by the photoreceptor cell ratio at the central retina 24 h and 7 days after MNU administration. Western-blot analysis demonstrated the expression levels of procaspase-9, -7, -3 and cleaved caspase-9, -7, -3 were upregulated, and PARP were downregulated both 24 h and 7 days after MNU injection. LBP treatment significantly decreased protein levels of procaspase and cleaved caspase, increased the level of PARP and cleaved PARP on 24 h and 7 days.

Conclusions

LBP inhibits MNU-induced rat photoreceptor cell apoptosis and protects retinal structure via the regulation of the expressions of PARP and caspase.