β-lactoglobulin stabilized nanemulsions—Formulation and process factors affecting droplet size and nanoemulsion stability
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- 作者：Ali Ali ; Ghozlene Mekhloufi ; ghozlene.mekhloufi@u-psud.fr" class="auth_mail" title="E-mail the corresponding author ; Nicolas Huang ; Florence Agnely
- 关键词：Sodium azide (PubChem CID ; 33557) ; Potassium phosphate dibasic (PubChem CID ; 24450) ; Potassium phosphate monobasic (PubChem CID ; 516951)
- 刊名：International Journal of Pharmaceutics
- 出版年：2016
- 出版时间：16 March 2016
- 年：2016
- 卷：500
- 期：1-2
- 页码：291-304
- 全文大小：1248 K
文摘
To avoid the toxicological concerns associated to synthetic surfactants, proteins might be an alternative for the stabilization of pharmaceutical nanoemulsions. The present study investigates the use of β-lactoglobulin (β-lg) to stabilize oil in water biocompatible nanoemulsions intended for a pharmaceutical use and prepared by high pressure homogenization (HPH). The effects of composition (nature and weight fraction of oil, β-lg concentration) and of process parameters (pressure and number of cycles) on the droplet size and on the stability of nanoemulsions were thoroughly assessed. The nanoemulsions prepared with β-lg at 1 wt% and with 5 wt% Miglyol 812 (the oil with the lowest viscosity) displayed a relatively small particle size (about 200 nm) and a low polydispersity when a homogenization pressure of 100 MPa was applied for 4 cycles. These nanoemulsions were the most stable formulations over 30 days at least. Emulsification efficiency of β-lg was reduced at higher homogenization pressures (200 MPa and 300 MPa). The effect of HPH process on the interfacial properties of β-lg was evaluated by drop shape analysis. This treatment had an effect neither on the interfacial tension nor on the interfacial dilatational rheology of β-lg at the Miglyol 812/water interface.