In vitro studies of biocompatible thermo-responsive hydrogels with controlled-release basic fibroblast growth factor

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• 作者：Ning Yang ; ^{summer_ningzi@163.com" class="auth_mail" title="E-mail the corresponding author} ; Hao Zhang ; Lu Shi ; Xiaoting Hu ; Yang He ; Li Chen ; ^{tjpuchenlis@163.com" class="auth_mail" title="E-mail the corresponding author} ; Qingsong Zhang ; Kongyin Zhao
• 关键词：Semi-IPN hydrogels ; N-isopropylacrylamide ; γ-Glutamic acid ; Controlled release ; BFGF
• 刊名：Reactive and Functional Polymers
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：100
• 期：Complete
• 页码：73-82
• 全文大小：1977 K

文摘

In this study, poly (γ-glutamic acid) with 72% sulfonated carboxyl groups and poly(N-isopropylacrylamide) (γ-PGA-S72/PNIPAAm) semi-interpenetrating network (semi-IPN) hydrogels were developed and used as a soft–wet platform for cell harvesting and controlled release of basic fibroblast growth factor (bFGF). The physical and chemical properties of the γ-PGA-S72/PNIPAAm semi-IPN hydrogels were systemically investigated. Furthermore, the specific binding and controlled release of growth factor were confirmed by enzyme-linked immunosorbent assay and the released bFGF shown to promote NIH-3T3 cell proliferation in 7 days. Cells could spontaneously detach from γ-PGA-S72/PNIPAAm semi-IPN hydrogels without enzyme treatment. This work demonstrates that the γ-PGA-S72/PNIPAAm semi-IPN08 hydrogel may be an excellent carrier for avoiding the sudden release and low bioactivity of growth factors in culture systems.