l-tryptophan and dipeptide derivatives for supercoiled plasmid DNA purification
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- 作者：Tiago Santosa ; 1 ; Josué ; Carvalhoa ; 1 ; Marta C. Corvob ; c ; Eurico J. Cabritab ; J.A. Queiroza ; C. Cruza ; carlacruz@fcsaude.ubi.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：NMR ; nuclear magnetic resonance ; STD ; Saturation Transfer Difference ; HR-MAS ; high resolution magic angle spinning ; SPR ; surface plasmon resonance ; 5´ ; -GMP ; guanosine 5&prime ; -monophosphate ; 3´ ; -AMP ; adenosine 5&prime ; -monophosphate ; 5´ ; -TMP ; thymidine 5&prime ; -monophosphate ; 5´ ; -CMP ; cytidine 5&prime ; -monophosphate ; sc ; supercoiled ; oc ; open-circular ; ln ; linear ; pDNA ; plasmid DNA ; E.coli ; Escherichia coli ; DCC ; N ; N&prime ; -dicyclohexyl carbodiimide ; BOC ; tertbutoxycarbonyl
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：87
- 期：Complete
- 页码：385-396
- 全文大小：1987 K
文摘
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We report the use of l-tryptophan and its derivatives for supercoiled purification.
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Three chromatographic supports have been synthetized and characterized.
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The mode of interaction with 5′-mononucleotides is mainly hydrophobic.
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The overall affinity between l-tryptophan derivatives is high with supercoiled.
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A purification strategy with l-tryptophan support is proposed.