Applying DNA affinity chromatography to specifically screen for sucrose-related DNA-binding transcriptional regulators of Xanthomonas campestris
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- 作者：Lennart Leß ; meiera ; 1 ; Rabeaa S. Alkhateeba ; b ; Fabian Schultea ; b ; Tim Steffensa ; b ; Tobias Pascal Lokab ; 2 ; Alfred Pü ; hlerb ; Karsten Niehausa ; Frank-Jö ; rg Vorhö ; ltera ; b ; 3 ; frank@cebitec.uni-bielefeld.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Xanthomonas campestris pathovar campestris ; Pull-down assay ; DNA affinity purification ; Promoter fishing ; Regulation ; Virulence genes
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 August 2016
- 年：2016
- 卷：232
- 期：Complete
- 页码：89-98
- 全文大小：1070 K
文摘
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Pull-down assay to identify DNA-binding transcriptional regulators.
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Identification of four sucrose-related transcriptional regulators.
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Xanthomonas regulator Clp is linked to sucrose.
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Novel Xanthomonas transcriptional regulator gene xcc-b100_2791.