Production of recombinant dengue non-structural 1 (NS1) proteins from clinical virus isolates
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- 作者：Benediktus Yohana ; Puspa Wardhanib ; Aryatib ; Hidayat Trimarsantoa ; c ; R. Tedjo Sasmonoa ; sasmono@eijkman.go.id" class="auth_mail" title="E-mail the corresponding author
- 关键词：Dengue ; NS1 ; Recombinant protein ; Diagnostic
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：53-59
- 全文大小：860 K
文摘
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We expressed dengue NS1 proteins from Indonesia DENV clinical isolates.
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NS1 proteins were expressed using a site-specific recombination cloning method.
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The method can be used for producing NS1 using virus from other geographical regions with minimal modification.
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The purified NS1 proteins were antigenically active.
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The NS1 proteins produced can induced antibody response in mice.