High-throughput purification of recombinant proteins using self-cleaving intein tags
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- 作者：M.J. Coolbaugha ; M.J. Shakalli Tangb ; D.W. Woodb ; wood.750@osu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Intein tag ; High throughput protein purification ; Self-cleaving tag ; Elastin-like polypeptide ; Affinity methods ; Recombinant protein purification
- 刊名：Analytical Biochemistry
- 出版年：2017
- 出版时间：1 January 2017
- 年：2017
- 卷：516
- 期：Complete
- 页码：65-74
- 全文大小：1987 K
文摘
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Developed a method for high throughput protein purification based on self cleaving inteins.
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Simple, effective one step purification scaled down to 96-well plate format.
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Demonstrated good plate-to-plate and well-to-well reproducibility.