Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and Benzo[¦Á]pyrene

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• 作者：Eun-Ji Won ; Jae-Sung Rhee ; Kyung-Hoon Shin ; Jee-Hyun Jung ; Won Joon Shim ; Young-Mi Lee ; Jae-Seong Lee
• 关键词：Polychaete ; Perinereis nuntia ; Cytochrome P450 ; Water accommodated fraction ; WAF ; Benzo[¦Á]pyrene ; Antioxidant genes
• 刊名：Marine Environmental Research
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：90
• 期：Complete
• 页码：75-84
• 全文大小：2333 K

文摘

To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[¦Á]pyrene (B[¦Á]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[¦Á]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[¦Á]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P.?nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P.?nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.