文摘
To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[¦Á]pyrene (B[¦Á]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[¦Á]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[¦Á]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P.?nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P.?nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.