Molecular cloning and expression of Hedychium coronarium farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral and wounding/herbivory induced leaf volatile sesquiterpenoids

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• 作者：Jian-bin Lan ; Rang-cai Yu ; Yun-yi Yu ; Yan-ping Fan ; ^{fanyanping@scau.edu.cn}
• 关键词：FPPS ; farnesyl pyrophosphate synthase ; DMAPP ; dimethylallyl pyrophosphate ; FPP ; farnesyl pyrophosphate ; GC&ndash ; MS ; gas chromatography&ndash ; mass spectrometry ; GPP ; geranyl pyrophosphate ; IPP ; isopentenyl pyrophosphate ; DXP ; 1-deoxyxylulose-5-phosphate
• 刊名：Gene
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：518
• 期：2
• 页码：360-367
• 全文大小：1189 K

文摘

Farnesyl pyrophosphate synthase (FPPS EC 2.5.1.10) catalyzes the production of farnesyl pyrophosphate (FPP), which is a key precursor for many sesquiterpenoids such as floral scent and defense volatiles against herbivore attack. Here we report a new full-length cDNA encoding farnesyl diphosphate synthase from Hedychium coronarium. The open reading frame for full-length HcFPPS encodes a protein of 356 amino acids, which is 1068 nucleotides long with calculated molecular mass of 40.7 kDa. Phylogenetic tree analysis indicates that HcFPPS belongs to the plant FPPS super-family and has strong relationship with FPPS from Musa acuminata. Expression of the HcFPPS gene in Escherichia coli yielded FPPS activity. Tissue-specific and developmental analyses of the HcFPPS mRNA and corresponding volatile sesquiterpenoid levels in H. coronarium flowers revealed that the HcFPPS might play a regulatory role in floral volatile sesquiterpenoid biosynthesis. The emission of the FPP-derived volatile terpenoid correlates with strong expression of HcFPPS induced by mechanical wounding and Udaspes folus-damage in leaves, which suggests that HcFPPS may have an important ecological function in H. coronarium vegetative organ.