Mass spectrometry analysis shows the biosynthetic pathways supported by pyruvate carboxylase in highly invasive breast cancer cells

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• 作者：Phatchariya Phannasil^a ; Israr-ul H. Ansari^b ; Mahmoud El Azzouny^c ; Melissa J. Longacre^b ; Khanti Rattanapornsompong^a ; Charles F. Burant^c ; Michael J. MacDonald^b ; Sarawut Jitrapakdee^a ; ^{sarawut.jit@mahidol.ac.th}
• 关键词：PC ; pyruvate carboxylase ; MS ; mass spectrometry
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1863
• 期：2
• 页码：537-551
• 全文大小：1306 K
• 卷排序：1863

文摘

Stable suppression of PC in breast cancer cells lowers their proliferation rate. Suppression of PC does not affect glycolysis or cellular energy production. Suppression of PC impaired mitochondrial anaplerosis from glucose and glutamine. Impaired anaplerosis lowers synthesis of amino acids, nucleotides and fatty acids.