Site-saturation engineering of proline 474 in pyruvate carboxylase from Rhizopus oryzae to elevate fumaric acid production in engineered Saccharomyces cerevisiae cells

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• 作者：Guoqiang Xu^a ; ^b ; ^c ; Manzhen Wu^a ; ^b ; Linghuo Jiang^a ; ^b ; ^c ; ^{linghuojiang@jiangnan.edu.cn}
• 关键词：Pyruvate carboxylase ; Fumaric acid ; Saccharomyces cerevisiae ; Site-saturation engineering
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：117
• 期：part_PB
• 页码：36-42
• 全文大小：1622 K
• 卷排序：117

文摘

The strain pdc1adh1fum1 can produce fumaric acid with low ethanol titer. RoPYC was found to be more beneficial for fumaric acid production than ScPYC2. The P474N mutant’s overexpression led to a significant higher levels of fumaric acid. Supplementation of 56 μg l−1 biotin produced the best yield of fumaric acid.