Analysis of reference gene stability after Israeli acute paralysis virus infection in bumblebees Bombus terrestris

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• 作者：Jinzhi Niu ; Kaat Cappelle ; Joachim R. de Mir ; a ; Guy Smagghe ; Ivan Meeus
• 关键词：Gene expression ; Bee virus ; RT-qPCR ; Dicistroviruses ; qBasePLUS
• 刊名：Journal of Invertebrate Pathology
• 出版年：January, 2014
• 年：2014
• 卷：115
• 期：Complete
• 页码：76-79
• 全文大小：384 K

文摘

To date, there are no validated internal reference genes for the normalization of RT-qPCR data from virus infection experiments with pollinating insects. In this study we evaluated the stability of five candidate internal reference genes: elongation factor-1-alpha (ELF1伪), peptidylprolyl isomerase A (PPIA), 60S ribosomal protein L23 (RPL23), TATA-binding protein (TBP) and polyubiquitin (UBI), in relation to Israeli acute paralysis virus (IAPV) infection of Bombus terrestris. We investigated the stability of these genes: in whole bodies and individual body parts, as well as in whole bodies collected at different time intervals after infection with IAPV. Our data identified PPIA as the single, most-optimal internal reference gene and the combination of PPAI-RPL23-UBI as a fully-sufficient multiple internal reference genes set for IAPV infection experiments in B. terrestris.