Low molecular weight fucoidan ameliorates streptozotocin-induced hyper-responsiveness of aortic smooth muscles in type 1 diabetes rats

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• 作者：Zhengyang Liang^a ; ¹ ; ^{nxsmzwnemhds@126.com" class="auth_mail" title="E-mail the corresponding author} ; Yuanyuan Zheng^a ; ¹ ; ^{zhengyy99@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jing Wang^b ; ^{jingwang@qdio.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Quanbin Zhang^b ; ^{qbzhang@qdio.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Shuang Ren^a ; ^{renshuang12@126.com" class="auth_mail" title="E-mail the corresponding author} ; Tiantian Liu^a ; ^{liutiantian008@126.com" class="auth_mail" title="E-mail the corresponding author} ; Zhiqiang Wang^a ; ^{zhiqiang3250101@126.com" class="auth_mail" title="E-mail the corresponding author} ; Dali Luo^a ; ^{luodl@ccmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Fucoidan ; Diabetes ; Vascular smooth muscles ; Aorta ; Hypertension
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：15 September 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：341-349
• 全文大小：1478 K

文摘

Low molecular weight fucoidan (LMWF) was prepared from Laminaria japonica Areschoug, a popular seafood and medicinal plant consumed in Asia. Chinese have long been using it as a traditional medicine for curing hypertension and edema.

Aim of the study

This study was intent to investigate the possible beneficial effect of LMWF on hyper-responsiveness of aortic smooth muscles instreptozotocin (STZ)-induced type 1 diabetic rats.

Materials and methods

Sprague-Dawley rats were made diabetic by injection of STZ, followed by the administration of LMWF (50 or 100 mg/kg/day) or probucol (100 mg/kg/day) for 12 weeks. Body weight, blood glucose level, basal blood pressure, serum lipid profiles, oxidative stress, prostanoids production, and vasoconstriction response of endothelium-denuded aorta rings to phenylephrine were measured by Real time-PCR, Western blots, ELISA assay, and force myograph, respectively.

Results

LMWF (100 mg/kg/day)-treated group showed robust improvements on STZ-induced body weight-loss, hypertension, and hyperlipidaemia as indicated by decreased serum level of total cholesterol, triglyceride, and low density lipoprotein cholesterol; while probucol, a lipid-modifying drug with antioxidant properties, displayed mild effects. In addition, LMWF appreciably ameliorated STZ-elicited hyper-responsiveness and oxidative stress in aortic smooth muscles as indicated by decreased superoxide level, increased glutathione content and higher superoxide dismutase activity. Furthermore, administration with LMWF dramatically prevented cyclooxygenase-2 stimulation and restored the up-regulation of thromboxane synthase and down-regulation of 6-keto-PGF_1α (a stable metabolic product of prostaglandin I₂) in the STZ-administered rats.

Conclusion

This study demonstrates for the first time that LMWF can protect against hyperlipidaemia, hypertension, and hyper-responsiveness of aortic smooth muscles in type 1 diabetic rat via, at least in part, amelioration of oxidative stress and restoration of prostanoids levels in aortic smooth muscles. Therefore, LMWF can be a potential adjuvant treatment against cardiovascular complications in type 1 diabetes.