In vitro and in vivo antitumor activity of oridonin nanosuspension

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• 作者：Haiyan Lou ; Xiumei Zhang ; Lei Gao ; Feifei Feng ; Juying Wang ; Xinbing Wei ; Zongqin Yu ; Dianrui Zhang ; Qiang Zhang
• 关键词：Oridonin ; Nanosuspension ; Antitumor ; Cytotoxicity
• 刊名：International Journal of Pharmaceutics
• 出版年：2009
• 出版时间：8 September 2009
• 年：2009
• 卷：379
• 期：1
• 页码：181-186
• 全文大小：834 K

文摘

The aim of the present study was to evaluate the antitumor activity of an oridonin (ORI) nanosuspension relative to ORI solution both in vitro and in vivo. ORI nanosuspension with a particle size of 897.2 ± 14.2 nm was prepared by the high pressure homogenization method (HPH). MTT assay showed that ORI nanosuspension could significantly enhance the in vitro cytotoxicity against K562 cells compared to the ORI solution, the IC₅₀ value at 36 h was reduced from 12.85 μmol/L for ORI solution to 8.11 μmol/L for ORI nanosuspension. Flow cytometric analysis demonstrated that the ORI nanosuspension also induced a higher apoptotic rate in K562 cells compared to ORI solution. In vivo studies in a mouse model of sarcoma-180 solid tumors demonstrated significantly greater inhibition of tumor growth following treatment with ORI nanosuspension than ORI solution at the same dosage. The mice injected with ORI nanosuspension showed a higher reduction in tumor volume and tumor weight at the dose of 20 mg/kg compared to the ORI solution (P < 0.01), with the tumor inhibition rate increased from 42.49 % for ORI solution to 60.23 % for the ORI nanosuspension. Taken together, these results suggest that the delivery of ORI in nanosuspension is a promising approach for the treatment of the tumor.