Cloning, expression and localization of DacaCSP2 and DacaCSP3 during different reproductive stages in Daphnia carinata
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- 作者：Haixia Li1 ; Yang Yang1 ; Guorong Xu ; Donglei Wu ; Weiwei Lv ; Qicheng Jiang ; Yunlong Zhao ; ylzhao@bio.ecnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：CSPs ; chemosensory proteins ; DacaCSP2 ; Daphnia carinata chemosensory protein 2 ; DacaCSP3 ; Daphnia carinata chemosensory protein 3 ; ISH ; in situ hybridization ; OS-D ; olfactory specific protein D ; qPCR ; quantitative polymerase chain reaction ; pI ; isoelectric point ; MW ; molecular weight ; MEGA ; Molecular Evolutionary Genetics Analysis ; UTR ; untranslated region ; An2 ; second antenna ; T ; thoracic limbs ; O ; ovary ; R ; rectum ; S ; summer eggs
- 刊名：Gene
- 出版年：2016
- 出版时间：10 May 2016
- 年：2016
- 卷：582
- 期：1
- 页码：59-68
- 全文大小：2342 K
文摘
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We cloned full-length cDNA of two chemosensory genes (CSP2/3) from Daphnia carinata.
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CSP2/3 protein levels varied between males, and sexual or parthenogenetic females.
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CSP2/3 are expressed in the ovaries, summer eggs, thoracic limbs, rectum and second antennae.
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CSP2/3 may be the trigger for females to transform into males.
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CSP2/3 mRNA expression levels increase in response to temperature changes.