Evaluation of immunogenicity and protective efficacy of Actinobacillus pleuropneumoniae HB04C⁻ mutant lacking a drug resistance marker in the pigs

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• 作者：Weicheng Bei ; Qigai He ; Rui Zhou ; Lin Yan ; Hongliang Huang ; Huanchun Chen
• 关键词：Actinobacillus pleuropneumoniae ; RTX toxin ; Live attenuated vaccine
• 刊名：Veterinary Microbiology
• 出版年：2007
• 出版时间：15 November 2007
• 年：2007
• 卷：125
• 期：1-2
• 页码：120-127
• 全文大小：293 K

文摘

Previously, we reported the construction and characterization of a genetically defined Actinobacillus pleuropneumoniae (A. pleuropneumoniae) apxIIC gene mutant, HB04C⁻, which conferred protection to mice against infection with A. pleuropneumoniae. In this study, we further evaluated HB04C⁻ for safety and its ability to elicit protective immunity in pigs. It was demonstrated that a dose of 2 × 10⁸ CFU HB04C⁻ was safe to the pigs via intranasal or intramuscular injection. Immunization with a dose of 2 × 10⁸ HB04C⁻ by both intranasal and intramuscular routine could yield equal protective efficacy and elicited significant protection against experiment challenge with homologous or heterologous serotypes of a virulent A. pleuropneumonia. Taken together, HB04C⁻ might serve as a promising vaccine candidate against infection with A. pleuropneumoniae.